Project ID: HU09-0009-A2-2013

 analysis of bioactive compounds in horseradish (Armoracia); development of analytical methods for the quantification of the above bioactive compounds; analysis of the antimicrobial effects of the essential oil made from horseradish; development of a large scale isolation technology for the production of essential oil from the secondary roots of horseradish; adaptability analysis of the (fungicide and/or algaecide) liquid with biocide effects made from the essential oil; development of a (fungicide and/or algaecide) product with biocide effects containing

horseradish essential oil; characterization of the by-products of essential oil production; development of methods suitable for measuring peroxidase and producing peroxidase in a clean form; activities concerning industrial property protection.



  Objectives of the project:


The main objective of the project is to make innovative products containing natural compounds from potentially dangerous vegetable waste using environmentally friendly technologies. The participants wish to conduct the necessary research and development by themselves.


The aim is to utilize and make harmless the potentially dangerous by-products – especially the secondary roots – which contain isothiocyanates, as well as the roots that are of unmarketable quality and/or size, together with the above-ground parts of horseradish intensively cultivated in a large area around Debrecen in a way that results in new useful products usable in food industry, agriculture and other industries. The potentially dangerous isothiocyanates can be extracted and cleanly produced from the plant with suitable methods.


In the project we plan to utilize vegetable waste with the help of environmentally friendly technologies and methods developed by ourselves for the isolation of one valuable component. Our first method corresponds to the production of essential oil (isothiocyanate mixture), that is we divide the vegetable waste into isothiocyanates, which are dangerous in high concentration but can be potentially used in food and chemical industries as well as in agriculture, and other easily utilizable and non-hazardous by-products. In the course of our second method, a protein fraction rich in peroxidase enzyme is produced from the vegetable waste.


Both isolation methods will be developed. Experience gained during the research and development process together with market conditions will determine the proportion of the two products.


The region around Debrecen (Eastern Hungary) is one of the largest horseradish growing areas in the world in respect of both quality and quantity. This region has all the necessary features to produce high quality horseradish; the flavour and scent of the product are excellent, production is effective. The roots are not only used as spice; several new ways of utilization have appeared recently (e.g. preservation). The root is a functional food; its bioactive compounds are isothiocyanates, which are produced when the enzymes of the plant decompose glucosinolates. These compounds are very likely to have cancer preventive, antimicrobial and other effects that are important to different industries.


However, horseradish cultivation generates large quantities of vegetable waste. Several hundred tons of secondary roots, small sized (practically unmarketable) roots and green mass, which are not utilized at all, are produced in the Debrecen region every year. The secondary roots must be collected to prevent unwanted reproduction. The leaves and the secondary roots in particular cannot be directly utilized as fodder due to their isothiocyanate contents and composting them is also problematic. Inhaled or consumed in large concentration, isothiocyanates have harmful effect on mammals, fish and several other organisms. Owing to the high-efficiency plant production methods used in the developed world, making different agricultural wastes harmless poses serious challenges. New effective methods, by which it becomes possible to make the waste harmful, are necessary for handling this huge quantity of waste. Our aim is to find a solution for the best possible utilization of these vegetable wastes.


The main purpose of this project is the utilization of the vegetable waste of horseradish in a way that results in new useful products utilizable in food industry, agriculture and other industries. With suitable methods, the potentially dangerous isothiocyanates can be cleanly produced from the plant. The Department of Botany of the University of Debrecen has been researching technologies for the production of essential oil and in cooperation with Kelet Product Zrt. it has started basic researches on horseradish. Kelet Product Zrt. has launched investments aiming to produce essential oil or other useful products from horseradish using methods where the by-products are harmful and easily utilizable.


In the frame of this project, the Hungarian participants and their Norwegian partner intend to carry out the experimental development of products which are new even from the viewpoint of industrial property protection and contain only natural compounds.


These products may be useful for several industries. The planned experimental development contains the following:

Development and production of natural bioactive

compound based products from vegetable waste of

horseradish using environmentally friendly technology